The respective rates of overall survival with bevacizumab and placebo were 72.4 percent and 66.3 percent at 1 year and 33.9 percent and 30.1 percent at 24 months . Various other Clinical Measures All of the patients were required to complete quality-of-lifestyle questionnaires. Over 12 months of treatment, the %age of sufferers who completed all of the quality-of-lifestyle questionnaires at an evaluation ranged from 74 to 91 percent. In the prespecified primary analysis, deterioration-free survival was significantly longer among individuals in the bevacizumab group than among those in the placebo group for all five prespecified scales ; similarly, in exploratory analyses, deterioration-free survival was considerably longer among patients in the bevacizumab group for all 21 nonprespecified scales .All serum samples had been depleted of IgG antibodies by using Eurosorb reagent , according to the process. The screening dilution was 1:40. Recombinant ELISA A recombinant ELISA assay was predicated on soluble MERS-CoV spike proteins S1 domain expressed in HEK-293T cells.11 This test was developed with the use of samples obtained from little groups of individuals and camels in preliminary studies.7,12 The dilution of human being serum samples in this test was 1:100. Additional technical details are provided in Section 1 in the Supplementary Appendix, obtainable with the full text of this article at NEJM.org. Neutralization Testing PRNT was performed as described previously.8 The access dilution in log2-dilution series was 1:10.